Amino Acid Analysis Protocols

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About this book

Amino acid analysis is widely used in biotechnology, biomedical, and food analysis laboratories. Amino Acid Analysis Protocols constitutes a major collection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Since results depend heavily on the quality of the sample, most contributors have devoted a section to sample preparation, particularly to the collection and storage of bodily fluids. A new method for desalting samples prior to hydrolysis is also provided. Each method is described in step-by-step detail to ensure successful experimental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems.
Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

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Table of contents (18 protocols)

Front Matter

Amino Acid Analysis

• Margaret I. Tyler

Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory

• Frank D. Macchi, Felicity J. Shen, Rodney G. Keck, Reed J. Harris

Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis

• Li Zhang, Nancy Denslow

Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate

• Steven A. Cohen

Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent)

• Sunil Kochhar, Barbara Mouratou, Philipp Christen

The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection

• C. David Forster, Charles A. Marsden

Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids

• Petr Jandik, Christopher Pohl, Victor Barreto, Nebojsa Avdalovic

Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane

• Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno, Kimie Murayama

Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples

• Hiroyuki Kataoka, Sayuri Matsumura, Shigeo Yamamoto, Masami Makita

Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization

Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography

• Kang-Lyung Woo

Amino Acid Measurement in Body Fluids Using PITC Derivatives

• Roy A. Sherwood

Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis of Phenylthiocarbamyl Derivatives

• Margareta Stark, Jan Johansson

Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection

• Hiroyuki Kataoka, Norihisa Sakiyama, Yukizo Ueno, Kiyohiko Nakai, Masami Makita

Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection

• Hiroyuki Kataoka, Kiyomi Takagi, Hirofumi Tanaka, Masami Makita

Capillary Electrophoretic Determination of 4-Hydroxyproline

• Qingyi Chu, Michael Zeece

Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization

• Isabella Fermo, Rita Paroni

Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins

• Malladi Prabhakaram, Beryl J. Ortwerth, Jean B. Smith

Back Matter

Reviews

"Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis."-Doody's Health Sciences Book Review Journal

". the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis."- Today's Life Science

". This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories. This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques." - Clinical Chemistry

"Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids." - Journal of Chromatographic Science

Editors and Affiliations

Proteome Systems Ltd, North Ryde, Australia

Bibliographic Information

• Book Title : Amino Acid Analysis Protocols
• Editors : Catherine Cooper, Nicolle Packer, Keith Williams
• Series Title : Methods in Molecular Biology
• DOI : https://doi.org/10.1385/1592590470
• Publisher : Humana Totowa, NJ
• eBook Packages : Springer Protocols
• Copyright Information : Humana Press 2000
• Hardcover ISBN : 978-0-89603-656-7 Published: 05 September 2000
• Softcover ISBN : 978-1-61737-117-2 Published: 10 November 2010
• eBook ISBN : 978-1-59259-047-6 Published: 05 February 2008
• Series ISSN : 1064-3745
• Series E-ISSN : 1940-6029
• Edition Number : 1
• Number of Pages : XI, 265
• Topics : Biochemistry, general